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Index > Protein center > FGF2(Gene name) > Human
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  • FGF2 (Gene name),
  • Heparin-binding growth factor 2 (Protein name ),  FGF2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    FGF2(FGFB);
    Protein name:
    Heparin-binding growth factor 2(HBGF-2);
    Alternative:
    Heparin-binding growth factor 2(HBGF-2);Basic fibroblast growth factor(bFGF);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2.
    Function:
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
    Subcellular Location:
    Secreted Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.
    Protein Attributes:
    Sequence length:
    288
    Sequence:
    50:
    MVGVGGGDVE | DVTPRPGGCQ | ISGRGARGCN | GIPGAAAWEA | ALPRRRPRRH | 
    100:
    PSVNPRSRAA | GSPRTRGRRT | EERPSGSRLG | DRGRGRALPG | GRLGGRGRGR | 
    150:
    APERVGGRGR | GRGTAAPRAA | PAARGSRPGP | AGTMAAGSIT | TLPALPEDGG | 
    200:
    SGAFPPGHFK | DPKRLYCKNG | GFFLRIHPDG | RVDGVREKSD | PHIKLQLQAE | 
    250:
    ERGVVSIKGV | CANRYLAMKE | DGRLLASKCV | TDECFFFERL | ESNNYNTYRS | 
    288:
    RKYTSWYVAL | KRTGQYKLGS | KTGPGQKAIL | FLPMSAKS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    String:
    UniGene:
    Pfam:
    KEGG:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human HBGF-2
    Cat.:
    E0551b
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human HBGF-2
    Cat.:
    E0551h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Human HBGF-2
    Cat.:
    E0551r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Human HBGF-2
    Cat.:
    E0551m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human HBGF-2
    Cat.:
    E0551c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Human HBGF-2
    MSDS:
    Please sign in first.
    CLIA Kit for Human HBGF-2
    MSDS:
    Please sign in first.
    CLIA Kit for Human HBGF-2
    Cat.:
    U0551c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human HBGF-2
    Cat.:
    U0551r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human HBGF-2
    Cat.:
    U0551b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human HBGF-2
    Cat.:
    U0551h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human HBGF-2
    Cat.:
    U0551m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human HBGF-2
    Cat.:
    P0551Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human HBGF-2
    Cat.:
    P0551Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human HBGF-2
    Polyclonal Antibody for Human HBGF-2
    Cat.:
    P0551Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human HBGF-2
    Polyclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Monoclonal Antibody for Human HBGF-2
    Protein for Human HBGF-2
    Protein for Human HBGF-2
    Protein for Human HBGF-2
    Protein for Human HBGF-2
    Protein for Human HBGF-2
    Protein for Human HBGF-2

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤9.8%

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    Recovery
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    Recovery was determined by spiking various levels of FGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    92-100

    Plasma

    98

    96-107

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-102%

    94-101%

    92-104%

    90-103%

    EDTA plasma(n=5)

    94-103%

    92-102%

    94-102%

    91-107%

    heparin plasma(n=5)

    92-104%

     

    90-107%

    93-104%

    95-102%

     

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    References
    1. 1.
      "Human basic fibroblast growth factor: nucleotide sequence, genomic organization, and expression in mammalian cells."
      Abraham J.A. , Whang J.L. , Tumolo A. , Mergia A. , Fiddes J.C.
      Cold Spring Harb. Symp. Quant. Biol.51:657-668(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Human basic fibroblast growth factor: nucleotide sequence and genomic organization."
      Abraham J.A. , Whang J.L. , Tumolo A. , Mergia A. , Friedman J. , Gospodarowicz D. , Fiddes J.C.
      EMBO J.5:2523-2528(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1)
    3. 3.
      "High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons."
      Prats H. , Kaghad M. , Prats A.C. , Klagsbrun M. , Lelias J.M. , Liauzun P. , Chalon P. , Tauber J.P. , Amalric F. , Smith J.A. , Caput D.
      Proc. Natl. Acad. Sci. U.S.A.86:1836-1840(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4);PROTEIN SEQUENCE OF 126-145 (ISOFORMS 1/2/4);ALTERNATIVE INITIATION
      tissue: Hepatoma.
    4. 4.
      "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
      Goshima N. , Kawamura Y. , Fukumoto A. , Miura A. , Honma R. , Satoh R. , Wakamatsu A. , Yamamoto J. , Kimura K. , Nishikawa T. , Andoh T. , Iida Y. , Ishikawa K. , Ito E. , Kagawa N. , Kaminaga C. , Kanehori K. , Kawakami B. , more...
      Nat. Methods5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
    5. 5.
      NIEHS SNPs program
      Submitted (2007-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    6. 6.
      "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
      Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192
    9. 9.
      "Mutations in the 5' untranslated region of the FGF-2 gene."
      Handschug K. , Archoukieh E. , Glaeser C.
      Submitted (2003-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192
      tissue: Blood.
    10. 10.
      "Cloning and expression of cDNA encoding human basic fibroblast growth factor."
      Kurokawa T. , Sasada R. , Iwane M. , Igarashi K.
      FEBS Lett.213:189-194(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-288 (ISOFORM 1)
    11. 11.
      "Characterization of high-molecular-mass forms of basic fibroblast growth factor produced by hepatocellular carcinoma cells: possible involvement of basic fibroblast growth factor in hepatocarcinogenesis."
      Shimoyama Y. , Gotoh M. , Ino Y. , Sakamoto M. , Kato K. , Hirohashi S.
      Jpn. J. Cancer Res.82:1263-1270(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 94-107 AND 162-173;FUNCTION;BIOPHYSICOCHEMICAL PROPERTIES;TISSUE SPECIFICITY
      tissue: Hepatoma.
    12. 12.
      "Human amniotic tumor that induces new bone formation in vivo produces growth-regulatory activity in vitro for osteoblasts identified as an extended form of basic fibroblast growth factor."
      Izbicka E. , Dunstan C. , Esparza J. , Jacobs C. , Sabatini M. , Mundy G.R.
      Cancer Res.56:633-636(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 125-140 (ISOFORMS 1/2/4)
    13. 13.
      "A form of human basic fibroblast growth factor with an extended amino terminus."
      Sommer A. , Brewer M.T. , Thompson R.C. , Moscatelli D. , Presta M. , Rifkin D.B.
      Biochem. Biophys. Res. Commun.144:543-550(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 132-288 (ISOFORMS 1/2/4);PROTEIN SEQUENCE OF 132-148; 153-160; 165-247; 252-261 AND 267-288 (ISOFORMS 2/4)
      tissue: Hepatoma.
      tissue: Placenta.
    14. 14.
      "Amino-terminal sequence of a large form of basic fibroblast growth factor isolated from human benign prostatic hyperplastic tissue."
      Story M.T. , Esch F. , Shimasaki S. , Sasse J. , Jacobs S.C. , Lawson R.K.
      Biochem. Biophys. Res. Commun.142:702-709(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 135-155 (ISOFORMS 1/2/3/4)
    15. 15.
      Zhang H.J. , Zhang S.M. , Zhuang H.
      Submitted (2004-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 143-288 (ISOFORMS 1/2/3/4)
    16. 16.
      "Human brain-derived acidic and basic fibroblast growth factors: amino terminal sequences and specific mitogenic activities."
      Gimenez-Gallego G. , Conn G. , Hatcher V.B. , Thomas K.A.
      Biochem. Biophys. Res. Commun.135:541-548(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 143-172 (ISOFORMS 1/2/3/4)
    17. 17.
      "Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors."
      Gautschi P. , Frater-Schroeder M. , Boehlen P.
      FEBS Lett.204:203-207(1986) [PubMed] [Europe PMC] [Abstract]
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